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Food Testing >> Blog >> Catechins in tea

Catechins in Tea - The Antioxidant Power Behind Every Cup

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Introduction

Catechins are a class of naturally occurring polyphenolic compounds belonging to the flavonoid family, widely recognized for their potent antioxidant properties. They are abundant in tea (Camellia sinensis), particularly green tea, where they contribute not only to flavor and astringency but also to multiple health benefits. The four major catechins found in tea are epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), and epigallocatechin gallate (EGCG)—the latter being the most studied for its bioactive potential.

Catechins in Tea

Catechins are valued in the food and nutraceutical industry for their role in promoting cardiovascular health, weight management, cancer prevention, and antimicrobial activity. At Eurofins, we help clients assess and validate catechin content in tea and tea-based products to ensure compliance, quality, and consumer trust.

Why Catechins in Tea Must Be Tested

At Eurofins, we recognize the importance of testing catechins for:

  • Quality Assurance: Verifying catechin levels to support functional food and nutraceutical claims.
  • Consumer Safety: Preventing adulteration or mislabeling that could mislead consumers.
  • Regulatory Compliance: Meeting national and international labeling and health claim standards.
  • Product Differentiation: Helping brands establish their products as premium, high-antioxidant teas.
  • Stability and Shelf-life: Understanding catechin degradation during processing, packaging, and storage.

Catechins in Different Types of Tea

The following table highlights the typical catechin content across major tea types:

Tea Type

Major Catechins

Typical Content (mg/g dry weight)

Remarks

Green Tea

EGCG, EGC, ECG, EC

60–125

Highest catechin concentration due to minimal oxidation

White Tea

EGCG, EGC

40–80

Mildly processed, retains polyphenols

Oolong Tea

EGCG, ECG

30–60

Semi-oxidized, intermediate catechin levels

Black Tea

Theaflavins, Thearubigins (catechin derivatives)

10–30

Catechins largely oxidized into other polyphenols

Herbal Infusions

Traces

<10

Catechin levels vary, often lower than Camellia sinensis teas

Regulatory Requirements for Catechin Labeling and Claims

Parameter

FSSAI (India)

EU (European Union)

Regulatory Authority

Food Safety and Standards Authority of India (FSSAI)

European Union (Regulation EC No 1925/2006 amendment)

Catechin Content in Tea Leaves

9.0% to 19.0% total catechins (dry matter basis)

Not specifically defined for traditional tea leaves

EGCG Daily Intake Limit

Not explicitly specified for general tea

Max 800 mg EGCG/day from green tea extracts in supplements/fortified foods

Scope of Regulation

Tea leaves, black and green tea, tea infusions, extracts

Green tea extracts in supplements and fortified foods, not traditional tea infusions

Safety Concerns

Quality standards, safety from contaminants

Liver toxicity risk at high EGCG intake (>800 mg/day)

Labeling Requirements

Ingredients and composition must be declared

Warning label required on high-EGCG supplements advising max intake

Application for Supplements

Allowed with regulated catechin content

Allowed with max EGCG intake restrictions and warning labels

Types of Catechin Tests We Offer

Eurofins provides comprehensive analytical testing for catechins and related polyphenols in tea and tea-based products using validated global methods:

  • Total Polyphenols: Folin-Ciocalteu assay for overall antioxidant profile.
  • Individual Catechins (EGCG, ECG, EGC, EC): Quantified via HPLC, UPLC, and LC-MS/MS.
  • Degradation Products: Monitoring theaflavins, thearubigins, and gallic acid in processed teas.
  • Stability & Shelf-life Studies: Evaluating catechin retention during packaging and storage.
  • Bioactive Potency Testing: Antioxidant capacity using DPPH, ABTS, and FRAP assays.

Challenges in Catechin Testing

Catechin testing requires advanced techniques due to:

  • Complex Matrices: Tea contains caffeine, theaflavins, tannins, and other compounds that may interfere with analysis.
  • Degradation Sensitivity: Catechins are highly sensitive to pH, temperature, and light, leading to losses if not handled properly.
  • Batch-to-Batch Variation: Differences in geography, cultivar, and processing influence catechin levels.
  • Regulatory Stringency: Nutraceutical labeling laws demand precise quantification and standardized reporting.

At Eurofins, our labs use stabilized extraction protocols, internal standards, and globally harmonized chromatographic methods to ensure accurate and reproducible results.

Advanced Instrumentation for Catechin Analysis

Eurofins employs cutting-edge platforms for catechin testing:

  • HPLC-UV & UPLC: Gold-standard methods for separation and quantification of individual catechins.
  • LC-MS/MS: Provides superior sensitivity and specificity for trace catechin detection in complex matrices.
  • UV-Vis Spectrophotometry: Rapid total polyphenol assessment.
  • NMR Profiling: For detailed fingerprinting of tea authenticity and polyphenol composition.
  • Accelerated Shelf-Life Testing (ASLT): Simulates storage conditions to evaluate catechin stability over time.

How Our Testing Process Works

At Eurofins, we prioritize reliability, clarity, and turnaround efficiency:

  • Sample Collection: Clear guidelines on sample size, packaging, and shipping to avoid catechin degradation.
  • Testing: Conducted in ISO/IEC 17025-accredited labs with validated global methods.
  • TAT: Fast-track (24–48h) and standard options (3–5 working days).
  • Reports: Comprehensive results with regulatory guidance and stability insights.

Who Needs Catechin Testing?

Our services are ideal for:

  • Tea growers and processors ensuring quality and export readiness.
  • Nutraceutical companies marketing green tea extracts and supplements.
  • Beverage and functional food manufacturers highlighting antioxidant benefits.
  • Exporters/importers navigating EFSA, FDA, and FSSAI regulations.
  • Clean-label and wellness brands validating bioactive content claims.

Case Study: Ensuring Compliance for a Green Tea Supplement Brand

A nutraceutical client approached Eurofins to validate EGCG levels in green tea extract capsules. Our HPLC and LC-MS/MS analysis revealed discrepancies between the label claim and actual catechin content. With our corrective guidance, the client reformulated, achieved compliance with FSSAI and EFSA requirements, and successfully launched their product across global markets.

Why Choose Eurofins for Catechin Testing?

  • Global Expertise: Trusted across 50+ countries with 900+ laboratories.
  • Accreditations: NABL, ISO/IEC 17025, FSSAI-recognized.
  • State-of-the-Art Facilities: Advanced LC-MS/MS, UPLC, and NMR profiling platforms.
  • Expert Guidance: Food chemists, tea scientists, and regulatory experts on hand.
  • Integrated Services: From raw tea testing to finished nutraceutical validation.

Explore Our Services

  • Partner with Eurofins to ensure your tea and nutraceutical products are safe, compliant, and market-ready.
  • Contact our testing experts for guidance on catechin profiling and labeling compliance.
  • Schedule catechin testing today to meet your regulatory and consumer trust goals.

Enquiry now: https://www.eurofins.in/food-testing/enquire-now/