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Gene Synthesis

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Our Gene Synthesis Process

DNA Structure Optimization:

(when requested) Review of your construct for potential repeat or hairpin structures, G/C content and base distribution, and strategies for modifying the construct.

Codon Usage Optimization :

(when requested) Adaptation of the amino acid or DNA sequence to the codon usage of the target organism.

Construct Design and Oligo Synthesis:

Design and in-house synthesis of overlapping oligonucleotides to support the optimal gene construction method.

Gene Assembly:

Synthetic DNA oligonucleotides are assembled in order with subsequent strand closure employing LCR (Ligase Chain Reaction) and/or other PCR techniques.

Subcloning:

The completed gene is cloned into one or more standard or customer requested vectors.

DNA Sequence Verification of the Gene Construct:

The correct sequence is confirmed through DNA sequencing of both strands at our in-house sequencing lab. Your gene construct is guaranteed by Eurofins Genomics as 100% correct.

Gene Evolution Libraries

Gene Evolution Libraries are ideally suited for protein mutation screening Eurofins Genomics can place mutations in single or multiple positions within specific domains or through randomized base substitutions (incorporation of pre-selected base mixtures at designated positions). These Gene Evolution Libraries can be delivered as linear DNA or as cloned libraries.

Specifications

Standard Genes

  • GC content from 40-65%
  • Homopolymer stretches less than 20 bp; repeats do not exceed 20 bp
  • No critical hairpin structures

Gene Evolution Libraries

Gene Evolution Libraries are ideally suited for protein mutation screening Eurofins Genomics can place mutations in single or multiple positions within specific domains or through randomized base substitutions (incorporation of pre-selected base mixtures at designated positions). These Gene Evolution Libraries can be delivered as linear DNA or as cloned libraries.

Specifications

Standard Genes

  • GC content from 40-65%
  • Homopolymer stretches less than 20 bp; repeats do not exceed 20 bp
  • No critical hairpin structures

Complex Genes

  • GC content <40% or >65%
  • Long homopolymer stretches, long repeats, and/or secondary structure may be present
  • Review of your sequence by our scientists may provide tips to increase your experimental success

Delivery and Documentation

Each synthetic DNA gene is delivered as plasmid DNA (mini-scale preparation). Higher scales are available upon request. Gene Evolution Libraries are delivered as linear DNA or fully cloned libraries. Our documentation of your construct includes:

  • Quality Assurance Report - details the agreed project specifications
  • Complete plasmid description
  • Restriction map
  • DNA sequence verification data