Spacer, Linker, Base & Sugar Modifications

Spacer, linkers, base & sugar modifications and their structures

These oligos are characterised by

• guaranteed yields
  We guarantee minimum yields in OD
• maximum flexibility
  Benefit from 2 different purification options in 3different synthesis scales
• highest quality
  OD measurement and MALDI-TOF MS ensure highest demands

2'-Deoxyinosine [I]

2'-Deoxyinosine (INO) is a universal base that is less destabilising than mismatches found within a wobble or degenerated base

2'-Deoxyuridine [U]

2'-Deoxyuridine (URI) are often used as a substitute for dT.

5-Methyl-dC [5MedC]

5-Methyl-dC stabilises the DNA duplex due to an increase in the melting temperature during the replacement of the dC.

Amino-C6-dT [AmC6dT]

Amino C6-dT allows internal modifications within the oligonucleotides sequence.

Biotin-dT [BIOdT]

Biotin-dT can be used for an internal biotin modification (e.g. for hybridisation studies).

2',3'-dideoxyC [23ddC]

3'-ddC prevents extension by DNA polymerases and prevents degradation by 3'-exonucleases.

dSpacer [Spd]

The dSpacer is used to create a stable a basic site within an oligonucleotide.

Spacer C3 [SpC3]

Spacer C3 can be coupled to the 3'-end and internally of an oligonucleotide sequence. This can prevent e.g. the elongation of the oligo during a PCR without a major influence to its annealing properties. Oligos with C3 spacers are therefore ideal hybridisation probes in PCR reactions.

Spacer C3 can be coupled to the 3'-end and internally of an oligonucleotide sequence. This can prevent e.g. the elongation of the oligo during a PCR without a major influence to its annealing properties. Oligos with C3 spacers are therefore ideal hybridisation probes in PCR reactions.

C3 spacers structures

Wobble [WOB] (defined ratio)

The following IUB code is used to describe degenerated bases (wobbles). Wobbles are available with a defined and non defined ratio.

The following IUB code is used to describe degenerated bases (wobbles). Wobbles are available with a defined and non defined ratio.

Specification

What you can expect from our oligos with spacer, linkers, base and sugar modifications

Product specifications:

• Synthesis scales from 0.05 to 1.0 µmol
• Purification options: HPLC,
• Sequence lengths: 5-80 bases
• TAT: 3-4 weeks

² There is no OD guarantee for oligos <18 and >35 bases or for oligos with multiple modifications What you can expect from our oligos with spacer, linkers, base and sugar modifications

Product specifications:

• Synthesis scales from 0.01 to 1.0 µmol
• Purification options: HPSF, HPLC, PAGE
• Sequence lengths: 5-80 bases
• TAT: 3-4 weeks