JavaScript is disabled. Please enable to continue!

Mobile search icon
Food Testing >> Blog >> Herbal biomarkers - Bacopa and Ashwagandha

Herbal biomarkers - Bacopa and Ashwagandha

Sidebar Image

Introduction

The world of herbal medicine has been harnessing the therapeutic potential of various plants for centuries. In recent years, the demand for herbal remedies has surged, with a growing interest in natural alternatives for health and wellness. Bacopa (Bacopa monnieri) and Ashwagandha (Withania somnifera) are two notable herbs known for their medicinal properties. In this blog post, we explore the concept of herbal biomarkers and delve into the remarkable benefits of Bacopa and Ashwagandha as herbal biomarkers, shedding light on their applications, efficacy, and the science behind their therapeutic effects.

Herbal biomarkers - Bacopa

Understanding Herbal Biomarkers

Herbal biomarkers are specific compounds or molecules found in medicinal plants that are used to authenticate their identity, assess their quality, and evaluate their potency. These biomarkers serve as chemical fingerprints that provide crucial information about the plant's authenticity, purity, and therapeutic potential. By identifying and quantifying these biomarkers, researchers and manufacturers can ensure the consistency and effectiveness of herbal products.

Bacopa (Bacopa monnieri) as a Herbal Biomarker

Bacopa, also known as Brahmi, has a long history of use in traditional Ayurvedic medicine for its cognitive-enhancing and stress-reducing properties. The primary biomarkers in Bacopa are bacosides, specifically bacoside A and bacoside B. These compounds have been extensively studied for their neuroprotective, memory-enhancing, and anti-inflammatory effects. Bacopa's biomarkers serve as quality indicators, allowing manufacturers to verify the presence and concentration of these key compounds in herbal formulations.

Ashwagandha (Withania somnifera) as a Herbal Biomarker

Ashwagandha, often referred to as Indian ginseng, is renowned for its adaptogenic properties, promoting resilience and overall well-being. The primary biomarkers in Ashwagandha are withanolides, particularly withaferin A. These bioactive compounds have demonstrated potent antioxidant, anti-inflammatory, and stress-reducing effects. By analysing the levels of withanolides, manufacturers can ensure the potency and authenticity of Ashwagandha products, providing consumers with consistent and reliable herbal remedies.

 

Applications and Benefits

Application

Bacopa (Bacopa monnieri)

Ashwagandha (Withania somnifera)

Cognitive Enhancement

Bacopa has shown potential in enhancing cognitive function, improving memory, and supporting mental clarity.

Ashwagandha is believed to support cognitive function, memory, and focus, enhancing mental performance.

Stress Reduction

Bacopa exhibits anxiolytic properties, helping to reduce anxiety and promote a sense of calm and relaxation.

Ashwagandha is renowned for its adaptogenic properties, supporting stress reduction and resilience.

Neuroprotection

Bacopa has demonstrated neuroprotective effects, potentially protecting against neurodegenerative diseases.

Ashwagandha may have neuroprotective properties, supporting brain health and reducing cognitive decline.

Energy and Vitality Boost

Bacopa is believed to support overall vitality and energy levels, combating fatigue and promoting alertness.

Ashwagandha acts as an adaptogen, boosting energy and stamina, and promoting overall physical vitality.

Immune Support

Bacopa is associated with potential immune-modulating effects, supporting a healthy immune system.

Ashwagandha has been linked to immune-enhancing properties, promoting a robust immune response.

 

Importance of testing Herbal biomarkers

Importance of Testing Herbal Biomarkers

Bacopa (Bacopa monnieri)

Ashwagandha (Withania somnifera)

Authenticity Verification

Ensures the presence of specific biomarkers like bacosides

Confirms the presence of specific biomarkers like withanolides

Quality Assurance

Indicates the quality and purity of Bacopa products

Indicates the quality and purity of Ashwagandha products

Potency Assessment

Quantifies the concentration of key biomarkers for efficacy

Quantifies the concentration of essential biomarkers for desired effects

Standardization

Allows for consistency in Bacopa formulations

Enables consistent Ashwagandha product composition and effectiveness

Safety Assurance

Helps detect adulteration or substitution in Bacopa products

Helps detect adulteration or substitution in Ashwagandha products

Regulatory Compliance

Meets regulatory requirements for Bacopa-containing products

Ensures compliance with regulations for Ashwagandha-containing products

Consumer Confidence

Builds trust in Bacopa products by ensuring authenticity and quality

Enhances consumer trust through authentication and quality verification

 

Market overview

The biomarkers market has been experiencing substantial growth, with a value of USD 66.97 billion in 2022. This market is expected to exhibit a compound annual growth rate (CAGR) of 13.3% from 2023 to 2030. Several factors contribute to this growth, including the growing importance of companion diagnostics, the rising prevalence of cancer, increased investments in biomarker research, and significant innovations resulting from ongoing research.

.

Biomarkers market

 

Regulations and standards

  • In India, the regulation of herbal supplements is overseen by the Ministry of AYUSH (Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homeopathy). The AYUSH Ministry has set up a Good Manufacturing Practices (GMP) committee for herbal products, which is responsible for developing and implementing standards for the production and testing of herbal supplements.
  • The AYUSH Ministry also has a list of approved herbal drugs, which includes Bacopa and Ashwagandha. This list is updated regularly to ensure that only safe and effective herbal drugs are available on the market.
  • In addition to the AYUSH Ministry, there are also a number of other organizations that set standards for herbal supplements in India. These organizations include the National Institute of Standards and Technology (NIST), the Indian Pharmacopoeia Commission (IPC), and the Ayurvedic Pharmacopoeia of India (API).
  • The NIST has developed a set of standards for the testing of herbal supplements, which are used by many manufacturers in India. The IPC is responsible for developing and publishing the Indian Pharmacopoeia, which includes standards for the identity, purity, and quality of a wide range of herbal substances. The API is a compendium of Ayurvedic drugs, which includes standards for the production and testing of Ayurvedic herbal drugs


Testing methods

Testing methods for herbal biomarkers involve the identification and quantification of specific chemical compounds present in the herbs. In the case of Bacopa (Bacopa monnieri) and Ashwagandha (Withania somnifera), there are certain active compounds that are commonly used as biomarkers to assess the quality and authenticity of these herbs. Here are the testing methods for the biomarkers of Bacopa and Ashwagandha:

Herb

Biomarker

Testing Method

Bacopa

Bacosides

High-performance liquid chromatography (HPLC)

Bacopa

Alkaloids (e.g., brahmine, herpestine)

HPLC or other suitable methods

Ashwagandha

Withanolides

High-performance thin-layer chromatography (HPTLC) or HPLC

Ashwagandha

Alkaloids (e.g., withanine, somniferine)

HPLC or other suitable methods

 

Eurofins role in testing Bacopa and Ashwagandha

Eurofins Scientific is a global company that provides testing and analytical services, including testing of herbal products. While I don't have access to real-time information, Eurofins, being a prominent player in the testing industry, may offer various services related to the testing of herbal biomarkers in Bacopa and Ashwagandha. Here are some potential roles Eurofins could have in this context:

Method Development and Validation: Eurofins may develop and validate analytical methods for the identification and quantification of biomarkers in Bacopa and Ashwagandha. This involves establishing robust testing protocols using techniques such as HPLC, HPTLC, or other suitable methods to ensure accurate and reliable results.

Quality Control Testing: Eurofins might offer quality control testing services for Bacopa and Ashwagandha products. This can include testing samples to verify the presence and concentration of specific biomarkers, ensuring compliance with regulatory standards and product specifications.

Authenticity and Adulteration Testing: Eurofins could perform tests to authenticate Bacopa and Ashwagandha herbs and detect any potential adulteration. This may involve using various analytical techniques to compare the chemical profiles of the samples against reference standards, identifying any variations or contaminants.

Certificate of Analysis (CoA): Eurofins might provide a Certificate of Analysis for Bacopa and Ashwagandha products, indicating the test results, including the presence and concentration of biomarkers. The CoA serves as a document of quality assurance and can be important for manufacturers, retailers, and consumers.

Eurofins Analytical Services India, located in Bangalore, has achieved a significant milestone by receiving recognition from the US Food and Drug Administration (FDA) under the Laboratory Accreditation for Analyses of Foods (LAAF) program. This makes Eurofins the first laboratory in Asia to receive such recognition.

References

  1. https://www.grandviewresearch.com/industry-analysis/biomarkers-
  2. Yamada,K.Hung, P., Park, T. K., Park, P. J., & Lim, B. O. (2011).A comparison of the immunostimulatory effects of the medicinal herbs Echinacea, Ashwagandha and Brahmi. https://doi.org/10.1016/j.jep.2011.05.017
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185980/
  4. Dubey, T., & Chinnathambi, S. (2019). Brahmi (Bacopa monnieri): An ayurvedic herb against the Alzheimer's disease. Archives of Biochemistry and Biophysics, 676, 108153. https://doi.org/10.1016/j.abb.2019.108153