Importnace of Measuring Caffeine in Tea and Coffee

Introduction
Caffeine is one of the most widely consumed psychoactive substances in the world, naturally found in tea leaves and coffee beans. While it provides a much-loved boost in energy and alertness, the caffeine content in tea and coffee can vary significantly based on preparation, origin, and processing. Accurate testing of caffeine levels is vital for regulatory compliance, consumer transparency, and product standardisation.
What is Caffeine?
Caffeine is a natural alkaloid belonging to the methylxanthine family. It functions as a central nervous system stimulant and is known for:
- Enhancing alertness and concentration
- Reducing fatigue
- Improving mood and cognitive performance
- Acting as a mild diuretic
It is commonly consumed in the form of:
- Brewed coffee
- Black, green, and white teas
- Instant coffee and tea products
- Energy drinks and soft drinks
Concerns in the Food Industry
Despite its popularity, excessive caffeine intake can pose health risks, leading to concerns such as:
- Caffeine Sensitivity: Some individuals experience insomnia, jitteriness, or anxiety at low doses
- Overconsumption: Hidden caffeine in flavoured drinks, chocolates, and supplements may contribute to high daily intake
- Inconsistent Labelling: Unregulated or misleading claims can confuse consumers about actual caffeine content
- Health Risks in Vulnerable Populations: High intake during pregnancy or in children may pose developmental risks
Importance of Measuring Caffeine Content
As consumption of caffeinated beverages rises globally, precise quantification of caffeine becomes essential for several reasons. The table below outlines the importance of measuring caffeine:
Purpose |
Description |
Quality Assurance |
Ensures consistent caffeine levels across product batches |
Regulatory Compliance |
Meets national and international labelling and safety regulations |
Consumer Information |
Enables informed consumption, especially for sensitive groups like children and pregnant women |
Product Labelling |
Supports accurate caffeine content declaration for packaged drinks |
Standards and regulations
FSSAI Caffeine Standards for Coffee and Beverages
Product |
Caffeine Standard (on dry basis, unless specified) |
Roasted and Ground Coffee |
Not less than 1.0% by mass |
Decaffeinated Roasted and Ground Coffee |
Max 0.1% by mass |
Soluble Coffee Powder |
Not less than 2.8% by mass |
Decaffeinated Soluble Coffee Powder or Granules |
Max 0.3% by mass |
Coffee-Chicory Mixture |
Not less than 0.6% by mass |
Instant Coffee-Chicory Mixture |
Not less than 1.4% by mass |
Decaffeinated Roasted and Ground Coffee-Chicory Mixture |
Max 0.1% by mass |
Decaffeinated Instant Coffee-Chicory Mixture |
Max 0.3% by mass |
Carbonated Water |
Not exceeding 145 ppm |
Caffeinated Beverage (Carbonated and Non-Carbonated) |
145–300 mg per liter from all sources |
Non-Carbonated Water-Based Beverages (Non-Alcoholic) |
Not exceeding 145 ppm from all sources |
Labelling for Caffeinated Beverages |
“High Caffeine: X mg/serving size” (X = caffeine amount in mg per pack/serve) |
Regulatory Authority |
Caffeine Content Limit |
Notes |
ICMR |
Recommended daily intake: 300 mg |
Equivalent to approximately 2–3 cups of brewed coffee or 3–4 cups of tea |
European Union (EU) Regulation (EU) No 1169/2011 |
Labelling required if >150 mg/l in beverages |
Must state: "High caffeine content. Not recommended for children or pregnant or breast-feeding women" with caffeine in mg/100 ml |
Testing Protocols and Techniques
Testing Methods for Caffeine
- HPLC (High-Performance Liquid Chromatography): Gold standard for accurate quantification of caffeine in beverages and solids
Eurofins Testing Services for Caffeine Analysis
Eurofins offers comprehensive testing of caffeine in tea, coffee, and other food and beverage products. Their analytical services include:
- Quantification of caffeine content in brewed and instant teas and coffees
- Validation of product labelling for compliance with FSSAI, EFSA, and FDA standards
- Monitoring of caffeine levels in energy drinks, herbal products, and dietary supplements
- Stability studies to determine caffeine retention over shelf life.
- Custom method development for innovative or blended products
Eurofins’ globally accredited laboratories provide precise and reproducible results, helping manufacturers ensure compliance, transparency, and consumer trust.
Related Analytical Services Offered Alongside Caffeine Testing:
- Tannin and Polyphenol Profiling in tea and herbal extracts
- Adulterant Detection (e.g., synthetic caffeine in herbal drinks)
- Nutritional and Energy Content Testing
- Allergen and Contaminant Screening (pesticides, heavy metals, mycotoxins)