RRBS – Gene Methylation

Reduced representation bisulfite sequencing (RRBS) is an efficient method which enables analysis of DNA methylation on genome-scale at single-nucleotide level. Focusing on the genome containing CpG dinucleotides reduces DNA sequencing cost than whole genome. RRBS sequencing combined with bioinformatics analysis provides high resolution DNA methylation detection.

Workflow & Deliverables

Bioinformatics workflow for RRBS analysis:

• Quality check of raw reads
• Alignment of bisulfite sequencing reads against reference genome 
• Methylation calling
• mCs detection, methylation level calculation
• Differentially methylated regions (DMRs), Differentially Methylated Promoter (DMPs) detection
• Annotation of DMPs and DMRs for genomic regions
• Function enrichment analysis genes associated with DMR and DMP

Deliverables

• Quality filtration of reads
• Mapping of high quality reads to the reference genome
• Methylation profiling
• List of DMRs and DMPs with their annotation
• Differentially expressed DMRs and DMPs
• Gene ontology (GO) annotations of DMRs and DMPs related genes
• Compile report