O-Methyl-RNA / Chimerics

Chimeric oligos with RNA, DNA, PTO and 2'O-Methyl-RNA

The use of 2'O-Methyl-RNA in chimeric antisense oligos increases nuclease stability of the oligo.

Chimeric oligonucleotides consist of mixed DNA and RNA bases including methylated bases or phosphorthioate bounds (PTO) in various numbers and compositions.

O-Methyl-RNA / chimerics are delivered in 2ml screw cap tubes according to your specification:

• Hugh variety of RNA modifications
• HPLC purified
• Synthesis scales from 0.05 µmol up to 1.0 µmol
• Sequence lengths from 6 to 80 bases
• Quality control by OD measurement and MALDI-TOF MS
• Delivery occurs lyophilised in tube format

HPLC is required for purification of chimeric oligos. We recommend to perform a Na+ salt exchange with commercially available desalting columns (e.g. NAP or MicroSpin columns) before use in cells or live animals.

Specification:

What you can expect from our chimeric oligos

Product specifications:

• HPLC purified
• Synthesis scales from 0.05 µmol up to 1.0 µmol
• Minimum yields in OD
• Sequence lengths from 6 to 80 bases
• Quality control by OD measurement and MALDI-TOF MS
• TAT: 3-4 weeks
• Delivery: lyophilised in 2ml screw cap tubes

To differentiate between 2'O-Methyl-RNA, PTO, RNA and DNA bases enter your sequence as follows on the order page:

• DNA: ACGT
• RNA: (ACGU)
• PTO: A*C*(G*U)
• 2'-O-Methyl-RNA: [2OMeA], [2OMeC], [2OMeG], [2OMeU]