Circular RNA Sequencing

Circular RNAs (circRNAs) are non-coding RNAs with covalently closed loop without the 5′ cap and polyadenylation in the 3′ end. The play crucial roles in micro-RNA (miRNA) regulation, protein-protein interactions, regulation of parental gene transcription and disease progression in living organisms.

Workflow & Deliverables

Bioinformatics workflow for Circular lncRNA-seq analysis:

a.     Quality check of raw reads

b.     circRNA Identification 

c.     circRNA Expression Quantification

e.     Differential Expression Profiling

f.     Functional Analysis 

g.    Target Gene Prediction of CircRNAs