RNA modifications

Fluorescent dyes

5'

Int

3'

FAM [FAM]

x

-

-

Fluorescein [FLU]

-

-

x

Fluorescein-dT [FLUdT]

-

x

-

TET [TET]

x

-

-

HEX [HEX]

x

-

-

Cyanine3 [CY3]

x

-

-

TAMRA [TAM]

x

-

x

TAMRA-dT [TAMdT]

-

x

-

Cyanine5 [CY5]

x

-

-

Non fluorescent modifications

5'

Int

3'

Amino-C6 [AmC6]

x

-

-

Amino-C12 [AmC12]

x

-

-

Amino-C3/C6/C7 [AmC3] [AmC6] [AmC7]

-

-

x

Biotin [BIO]

x

-

x

Biotin-TEG [BIOTEG]

x

-

x

Cholesterol [CHOL]

-

-

x

Cholesteryl-TEG [CholTEG]

x

-

-

Phosphate [PHOS]

x

-

x

Spacer, linker, base & sugar modifications

5'

Int

3'

2'-Deoxyinosine [I]

-

x

x

2'-Deoxyuridine [U]

-

x

x

2',3' dideoxyC [23ddC]

-

-

x

5-Methyl-dC [5MedC]

-

x

x

Amino-C6-dT [AmC6dT]

-

x

-

Biotin-dT [BIOdT]

-

x

-

dSpacer [Spd]

-

x

-

Spacer C3 [SpC3]

-

x

x

Wobble [WOB] (defined ratio)

-

x

-

Dark quencher

5'

Int

3'

Dabcyl [DAB]

-

-

x

Dabcyl-dT [DABdT]

-

x

-

Eclipse [Eclip]

-

-

x

BHQ1 [BHQ1]

-

-

x

BHQ1-dT [BHQ1dT]

-

x

-

BHQ2 [BHQ2]

-

-

x

BBQ650 [BBQ650]

-

-

x

HPLC

>80%

Minimum Yield [OD]

0.5

2

2.5