DNA Modification
Rely on our long-year experience in modifying DNA oligos
Our modification center is built on 20 years of experience.
A wide range of common and alternative modifications in different synthesis scales and purification options are available for your individual applications:
Fluorescent dyes: We have wide range of dye combinations to provide all solution on time
Common reporter dyes such as FAM, HEX, TET, JOE, ROX
Cyanine dyes and Yakima Yellow
ATTO and Dyomics dyes
Dyes from Molecular Probes like Alexa Fluor and Bodipy dyes
Available fluorescent dyes and their characters
Answers to the following FAQs may help you to decide which fluorescent probes are right for your experiment:
How to choose the right dye?
One of the most common uncertainties when starting a new assay is how to select the best fluorescent label from all of the available options.
An optimal selection is based on reviewing the spectral properties of each dye, in relation to the instrument to be used. Each instrument has features to consider such as excitation source, optical filters, and sensitivity.
The most important dye characteristics to review when choosing an appropriate dye, are absorption- and emission spectra, the fluorescence Stokes shift, the extinction coefficient, pH-sensitivity, stability to photobleaching as well as the quantum yield.
Which criteria's are to be considered for choosing the right dye?
Absorption- and emission spectra of fluorescent dyes
The absorbance of a dye quantifies how much of visible or UV light is absorbed by it. The emission value shows at which wavelength a fluorescent dye sends light respective radiation out. Each fluorescent dye has its own absorption- and emission value (see schedule below). The choice of the right absorption-/ emission spectra of a dye depends on the individual assay as well on the type of instrument that is used.
Stokes shift
The difference in energy between the excitation and emission maximum is known as the Stokes shift. With fluorescent dyes, a large Stokes shift is often desirable when optical filters are used to separate exciting light and fluorescence emission.
Extinction coefficient
This value is a direct measurement of the dye's ability to absorb light. The ability to absorb light will clearly have an effect on the amount of light it is able to emit.
pH sensitivity
Some fluorophores are more sensitive to alkaline or acid pH conditions. For example, in alkaline solution above pH 9 some dye molecules could degrade. Other fluorophores are stable in alkaline as well as in acid pH ranges. One of the reasons for this is the structural characteristic of the molecules.
Stability to photobleaching
Photobleaching is the photochemical destruction of a fluorophore, which may impact the observation of fluorescent molecules, since they will eventually be destroyed by a constant light exposure. The loss of dye intensity respective of the quantum yield during experiments can produce erroneous results. However, it is important to consider your application and the instrument as well. For instance, stability to photobleaching is not as important for DNA sequencers and flow cytrometry as it is for fluorescence microscopy.
Fluorescence quantum yield
The quantum yield of a radiation induced process is the number of times that a defined event occurs per photon absorbed by the system. The fluorescence quantum yield (also quantum efficiency) gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed. Generally, the maximum fluorescence quantum yield is 1.0 (100%).
The quantum yield of fluorescent labels strongly depends on the current existing microenvironment such as pH-value, type of solution, concentration or temperature. Therefore specific quantum yields of single fluorescent dyes can not be revealed.
General characteristic of all available fluorescent dyes listed below
-
Alexa Flour 350 (Available at our US/Germany Facility): Please contact to our regional RMs
|
Alexa Fluor 350 |
|
|
Abs |
346 |
|
Em |
442 |
|
Ext. Coeff. |
19,000 |
|
Mol. Weight |
409.35 |
|
Alternative |
AMCA |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Multicolour detection |
|
Specification |
The general characteristics of Alexa Fluor dyes from Molecular Probes are: · Bright photo stable fluorescence · Good water solubility · pH insensitive between pH 4 - 10 · High quantum yield · Long fluorescence life time |
B. DY-415 dye based modification
|
DY-415 |
|
|
Abs |
418 |
|
Em |
467 |
|
Ext. Coeff. |
34,000 |
|
Mol. Weight |
552.00 |
|
Alternative |
Coumarin (DEAC) |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
FRET |
|
Specification |
DY-415 is part of the Dyomics fluorescent dyes produced by Dyomics GmbH. Dyomics dyes are characterised by: · High extinction coefficients · High fluorescence quantum yield · Large Stoke-Shift · High stability to photo-bleaching |
C. ATTO 425 Dye (Available at US/Germany site)
|
Dye |
ATTO 425 |
|
Abs |
436 |
|
Em |
484 |
|
Ext. Coeff. |
45,000 |
|
Mol. Weight |
499.00 |
|
Alternative |
Alexa Fluor 425 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Multicolour detection |
|
Specification |
ATTO 425 is a novel fluorescent label produced by ATTO-TEC GmbH. Characteristic features of the labels are · High fluorescence quantum yield · Large stokes shift · High photo stability and · Low molecular weight ATTO 425 is moderately hydrophilic. Its fluorescence can be excited efficiently in the range 405 - 455 nm. |
FAM [FAM]
|
Dye |
FAM |
|
Abs |
495 |
|
Em |
520 |
|
Ext. Coeff. |
83,000 |
|
Mol. Weight |
474.50 |
|
Alternative |
CY2 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
DNA sequencing / fragment length analysis |
|
Specification |
6-FAM (6-carboxyfluorescein) is the most commonly used fluorescent dye for various of applications. It has the same spectral characteristics than Fluoresceine and FITC and high quantum yields. FAM is compatible with most fluorescence detection systems and is typically used in the pH range 7.5-8.5. |
Fluorescein isothiocyanate [FITC] (DE/USA)
|
Dye |
FITC |
|
Abs |
495 |
|
Em |
520 |
|
Ext. Coeff. |
73,000 |
|
Mol. Weight |
505.00 |
|
Alternative |
CY2 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Multicolor detection |
|
Specification |
FITC is the abbreviation of fluoresceine isothiocyanate. The fluorescence of this molecule is very high and can be labelled at any position of an oligonucleotide. |
Fluoresceine [FLU] (DE/USA)
|
Dye |
Fluoresceine |
|
Abs |
495 |
|
Em |
520 |
|
Ext. Coeff. |
83,000 |
|
Mol. Weight |
504.00 |
|
Alternative |
DY-495 |
|
Labelling |
3' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, FRET |
|
Specification |
Fluoresceine is offered compared to FITC and FAM for the 3’ labeling of an oligo. It blocks the terminal OH-group of the oligomer. It is known as a dye with
Therefore this dye is displaced with more stable dyes like Oregon Green™ for applications such as FISH. 3’ Fluoresceine is used as the preferred donor dye in FRET systems or as an alternative to Digoxigenin and Biotin in immunological detection systems. |
Fluorescein-dT [FLUdT] (DE/USA)
|
Dye |
Fluorescein-Dt |
|
Abs |
494 |
|
Em |
522 |
|
Ext. Coeff. |
75,000 |
|
Mol. Weight |
472.71 |
|
Alternative |
DY-495 |
|
Labelling |
Int |
|
Purification |
HPLC |
|
Application |
Immunological detection |
|
Specification |
Fluorescein-dT is offered compared to Fluoresceine, FITC and FAM for labelling an oligo internally. This is done via the deoxy-group of the Thymidin base (-dT) and can therefore be used as modified primer. Fluorescein-dT is known as a dye with
Therefore this dye is displaced with more stable dyes like Oregon Green dyes for most of applications such as FISH. It can be used as alternative to Digoxigenin and Biotin-dT in immunological detection systems. |
ATTO 488 [ATTO488] (DE/USA)
|
Dye |
ATTO 488 |
|
Abs |
501 |
|
Em |
523 |
|
Ext. Coeff. |
90,000 |
|
Mol. Weight |
687.00 |
|
Alternative |
Cy2 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
ATTO 488 is a novel fluorescent label produced by ATTO-TEC GmbH. Characteristic features of the label are
The fluorescence is excited most efficiently For instance the 488 nm line of the Argon-Ion laser is very suitable for excitation. |
Oregon Green 488 [OG488] (DE/USA)
|
Dye |
Oregon Green 488 |
|
Abs |
496 |
|
Em |
524 |
|
Ext. Coeff. |
76,000 |
|
Mol. Weight |
510.00 |
|
Alternative |
Cy2 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Laser scanning microscopy |
|
Specification |
Oregon Green dyes are produced by Molecular Probes. Oregon Green 488 matches closely to the absorption spectra of fluoresceine but
|
Oregon Green 514 [OG514] (DE/USA)
|
Dye |
Orego Green 514 |
|
Abs |
506 |
|
Em |
526 |
|
Ext. Coeff. |
85,000 |
|
Mol. Weight |
609.40 |
|
Alternative |
Cy2 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Laser scanning microscopy |
|
Specification |
Oregon Green dyes are produced by Molecular Probes. Oregon Green 514 matches closely to the absorption spectra of fluoresceine but
|
Rhodamine Green [RGR] (DE/USA)
|
Dye |
Rhodamine Green |
|
Abs |
503 |
|
Em |
528 |
|
Ext. Coeff. |
74,000 |
|
Mol. Weight |
472.00 |
|
Alternative |
Cy2 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Laser scanning microscopy |
|
Specification |
Rhodamine Green from Molecular Probes are characterised by
The interaction between this modification and the oligo is reduced by a C7-Aminolink. |
TET [TET] (DE/USA)
|
Dye |
TET |
|
Abs |
521 |
|
Em |
536 |
|
Ext. Coeff. |
73,000 |
|
Mol. Weight |
612.30 |
|
Alternative |
CAL Fluor Gold 540 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
DNA sequencing / fragment length analysis |
|
Specification |
TET is the abbreviation of 5-Tetrachloro-Fluorescein, a phosphoramidite from Applied Biosystem. TET is an extremely stable dye. Its use is limited due to the close emission spectrum to FAM. TET is commonly used as 5’ fluorophore for Dual Labeled Probes and Molecular Beacons or as 5' labelled primer for different approaches on ABI instruments. |
ATTO 520 [ATTO520] (DE/USA)
|
Dye |
ATTO 520 |
|
Abs |
516 |
|
Em |
538 |
|
Ext. Coeff. |
110,000 |
|
Mol. Weight |
464.00 |
|
Alternative |
CAL Fluor Gold 540 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
ATTO 520 is a novel fluorescent label produced by ATTO-TEC GmbH related to the well-known dye Rhodamine 6G. Characteristic features of the label are
The dye is moderately hydrophilic. The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is an Argon-Ion laser using the 514 nm line. |
JOE [JOE] (DE/USA)
|
Dye |
JOE |
|
Abs |
520 |
|
Em |
548 |
|
Ext. Coeff. |
73,000 |
|
Mol. Weight |
603.40 |
|
Alternative |
CAL Fluor Gold 540 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
DNA sequencing |
|
Specification |
6-JOE is a 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluoresceine. 6-JOE has similar absorption and emission spectra to those of Rhodamine 6G. JOE is one of the traditional fluorophores used in automated DNA sequencing and as reporter dye for multiplex Real-Time qPCR. |
Yakima Yellow [YAKYE] (DE/USA)
|
Dye |
Yakima Yellow |
|
Abs |
530 |
|
Em |
549 |
|
Ext. Coeff. |
84,000 |
|
Mol. Weight |
654.30 |
|
Alternative |
VIC |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
DNA sequencing |
|
Specification |
Yakima Yellow is an exclusive dye made and sold under license from Epoch Biosciences, Inc. It offers researchers the best alternative to VIC of Applied Biosystems. Yakima Yellow is ideally suited to be used as a matched fluorophore in any Yakima Yellow labeled oligonucleotides are for resarch purpose only, and may not be used for commercial, clinical, diagnostic or any other use. |
Bodipy 530/550 [BO530] (DE/USA)
|
Dye |
Bodipy 530/550 |
|
Abs |
534 |
|
Em |
551 |
|
Ext. Coeff. |
77,000 |
|
Mol. Weight |
514.31 |
|
Alternative |
VIC |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
FRET |
|
Specification |
Bodipy 530/550 is part of the Bodipy family produced by Molecular Probes.
|
HEX [HEX] (DE/USA)
|
Dye |
HEX |
|
Abs |
535 |
|
Em |
556 |
|
Ext. Coeff. |
73,000 |
|
Mol. Weight |
681.20 |
|
Alternative |
VIC |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
DNA sequencing / fragment length analysis |
|
Specification |
HEX is the abbreviation of hexachloro-fluoresceine, a phosphoramidite from Applied Biosystem. HEX is commonly used as 5’ fluorophore for Dual Labeled Probes, Molecular Beacons or as 5' labeled primer for different approaches on ABI instruments. The phosphoramidite is already relatively unstable in the production. HEX labeled primers are very hydrophobe, affecting PCR efficiency. Thus, HEX is being substituted by Yakima Yellow for various of applications, already. |
Alexa Fluor 555 [Alexa555] (DE/USA)
|
Dye |
Alexa Fluor 555 |
|
Abs |
555 |
|
Em |
565 |
|
Ext. Coeff. |
150,000 |
|
Mol. Weight |
940.00 |
|
Alternative |
Cy3 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Multicolour detection |
|
Specification |
These are the specifications of Alexa Fluor 555 from Molecular Probes:
|
Bodipy TMR-X [BOTMRX] (DE/USA)
|
Dye |
Bodipy TMR-X |
|
Abs |
544 |
|
Em |
570 |
|
Ext. Coeff. |
56,000 |
|
Mol. Weight |
610.55 |
|
Alternative |
Quasar 570 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
FRET |
|
Specification |
Bodipy TMR-X is part of the Bodipy family produced by Molecular Probes. The characteristics of these modifications are
|
Cyanine3 [CY3] (DE/USA)
|
Dye |
Cyanine3 |
|
Abs |
550 |
|
Em |
568 |
|
Ext. Coeff. |
150,000 |
|
Mol. Weight |
553.7 |
|
Alternative |
Quasar 570 |
|
Labelling |
5', 3', intern |
|
Purification |
HPLC |
|
Application |
Microarray experiments |
|
Specification |
Cyanine3 is a fluorescent dye of the cyanine family. It is characterised by
The fluorescence lies in the deep yellow range. |
ATTO 550 [ATTO550] (DE/USA)
|
Dye |
ATTO 550 |
|
Abs |
554 |
|
Em |
576 |
|
Ext. Coeff. |
120,000 |
|
Mol. Weight |
691.00 |
|
Alternative |
NED |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
ATTO 550 is a novel fluorescent label produced by ATTO-TEC GmbH related to the dyes Rhodamine 6G and Rhodamine B. Characteristic features of the label are
The dye is moderately hydrophilic. The fluorescence is excited most efficiently in the range 540 - 565 nm. |
TAMRA [TAM]
|
Dye |
TAMRA |
|
Abs |
544 |
|
Em |
576 |
|
Ext. Coeff. |
90,000 |
|
Mol. Weight |
512.58 / 612.70 |
|
Alternative |
Rhodamine 6G |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
DNA sequencing / fragment length analysis |
|
Specification |
TAMRA is an abbreviation for carboxy-tetramethyl-rhodamine from Applied Biosystems. It can be used as fluorescent dye labelled on the 5’ end of the oligo, preferably used for fluorescence microscopy or for dye primer sequencing on ABI analyzers, but mostly used as 3’ quencher for short waved reporter dyes. |
Rhodamine Red [RRE] (DE/USA)
|
Dye |
Rhodamine Red |
|
Abs |
560 |
|
Em |
580 |
|
Ext. Coeff. |
129,000 |
|
Mol. Weight |
770.00 |
|
Alternative |
Rhodamine 6G |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Multicolour detection |
|
Specification |
Rhodamine Red from Molecular Probes is a useful second label in combination with other fluorescent dyes. It is characterised by
|
ATTO 565 [ATTO565] (DE/USA)
|
Dye |
ATTO 565 |
|
Abs |
563 |
|
Em |
592 |
|
Ext. Coeff. |
120,000 |
|
Mol. Weight |
608.00 |
|
Alternative |
PET |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
ATTO 565 is a novel fluorescent label produced by ATTO-TEC GmbH belonging to the class of Rhodamine dyes. Characteristic features of the label are
The dye is moderately hydrophilic. The fluorescence is excited most efficiently in the range 545-575 nm. |
ATTO RHO12 [ATTORHO12] (DE/USA)
|
Dye |
ATTO RHO12 |
|
Abs |
577 |
|
Em |
600 |
|
Ext. Coeff. |
120,000 |
|
Mol. Weight |
748.00 |
|
Alternative |
ROX, Texas Red |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR |
|
Specification |
ATTO RHO12 is a new rhodamine dye which belongs to the ATTO family from ATTO-TEC GmbH. Characteristic features of the label are
The dye is moderately hydrophilic. The fluorescence is excited most efficiently in the range of 550 - 580 nm. |
ROX [ROX] (DE/USA)
|
Dye |
ROX |
|
Abs |
575 |
|
Em |
602 |
|
Ext. Coeff. |
82,000 |
|
Mol. Weight |
632.78 |
|
Alternative |
Texas Red |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
DNA sequencing / fragment length analysis |
|
Specification |
ROX is the abbreviation for Carboxy-X-Rhodamine from Applied Biosystems. This ester is a red fluorescent dye that is coupled through an amino linker to the oligo, hence, ROX can not be combined with other ester based linkers. ROX is a member of the Rhodamine family. Compared to the fluoresceine family of dyes, Rhodamine dyes are more resistant to photo bleaching and stable over a wider pH range. The relatively long wavelength emission maximum for ROX makes it an attractive choice in multiplex detection systems. |
Texas Red [TxRed] (DE/USA)
|
Dye |
Texas Red |
|
Abs |
583 |
|
Em |
603 |
|
Ext. Coeff. |
116,000 |
|
Mol. Weight |
818.00 |
|
Alternative |
ROX |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
Texas Red is a commonly used red fluorescent dye from Molecular Probes. Its spectral properties are similar to ROX. TexasRed is only available as NHS ester and requires the attachment to an amino-modified oligonucleotide. It cannot be combined with other ester based linkers. Texas Red is commonly used as a third dye in specific experiments such as multiplex PCR due to their limited spectral overlap with other fluorescent dyes. It is also used as 5' reporter in FRET systems or just as alternative for ROX. |
Cyanine3.5 [CY35] (DE/USA)
|
Dye |
Cyanine3.5 |
|
Abs |
595 |
|
Em |
617 |
|
Ext. Coeff. |
150,000 |
|
Mol. Weight |
695.9 |
|
Alternative |
Alexa Fluor 594 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Microarray experiments |
|
Specification |
Cyanine3.5 is a fluorescent dye of the cyanine phosphoramidite family. It is characterised by
Cyanine3.5 is an alternative modification in multi-colour experiments. |
LightCycler 610 [LC610] (DE/USA)
|
Dye |
LightCycler 610 |
|
Abs |
590 |
|
Em |
610 |
|
Ext. Coeff. |
n.a. |
|
Mol. Weight |
761.48 |
|
Alternative |
Alexa Fluor 594 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
FRET, real-time PCR, qPCR |
|
Specification |
LightCycler Red 610 is an NHS ester supplied and patented by Roche Diagnostics GmbH. It is used for labelling the 5’-end for LightCycler probes to be used in LightCycler instruments. In addition, the 3’-end of the probe has to be blocked with C3 spacer or phosphate to avoid elongation during PCR. |
ATTO 594 [ATTO594] (DE/USA)
|
Dye |
ATTO 594 |
|
Abs |
590 |
|
Em |
627 |
|
Ext. Coeff. |
120,000 |
|
Mol. Weight |
903.00 |
|
Alternative |
Alexa Fluor 594 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Multicolour detection |
|
Specification |
ATTO 594 is a novel fluorescent label produced by ATTO-TEC GmbH belonging to the class of Rhodamine dyes. Characteristic features of the label are
ATTO 594 is very hydrophilic. The fluorescence is excited most efficiently in the range 580-615 nm. |
DY-480 XL [DY480] (DE/USA)
|
Dye |
DY-480 XL |
|
Abs |
500 |
|
Em |
630 |
|
Ext. Coeff. |
50,000 |
|
Mol. Weight |
612.68 |
|
Alternative |
- |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Microarray experiments |
|
Specification |
DY-480 XL is a part of the so called Megastoke family produced by Dyomics GmbH. Megastoke dyes exhibit a Additionally Megastoke dyes are also characterised by
|
DY-610 [DY610] (DE/USA)
|
Dye |
DY-610 |
|
Abs |
610 |
|
Em |
630 |
|
Ext. Coeff. |
80,000 |
|
Mol. Weight |
743.00 |
|
Alternative |
Alexa Fluor 610 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
DY-610 is part of the Dyomics fluorescent dyes produced by Dyomics GmbH. Dyomics dyes are characterised by
|
ATTO 610 [ATTO610] (DE/USA)
|
Dye |
ATTO 610 |
|
Abs |
615 |
|
Em |
634 |
|
Ext. Coeff. |
150,000 |
|
Mol. Weight |
488.00 |
|
Alternative |
Alexa Fluor 610 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
ATTO 610 belongs to a new generation of fluorescent labels from ATTO-TEC GmbH for the red spectral region. Characteristic features of this label are
ATTO 610 is moderately hydrophilic. The dye is very stable in pH-values up to pH 8.5. It slowly degrades at higher pH. The fluorescence is excited most efficiently in the range 595-625 nm. |
LightCycler 640 [LC640] (DE/USA)
|
Dye |
LightCycler 640 |
|
Abs |
625 |
|
Em |
640 |
|
Ext. Coeff. |
n.a. |
|
Mol. Weight |
824.68 |
|
Alternative |
- |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
FRET, real-time PCR |
|
Specification |
LightCycler Red 640 is an NHS ester supplied and patented by Roche Diagnostics GmbH. It is used for labeling the 5’-end for LightCycler probes to be used in LightCycler instruments. In addition, the 3’-end of the probe has to be blocked with C3 spacer or phosphate to avoid elongation during PCR. |
Bodipy 630/650 [BO630] (DE/USA)
|
Dye |
Bodipy 630/650 |
|
Abs |
625 |
|
Em |
640 |
|
Ext. Coeff. |
101,000 |
|
Mol. Weight |
662.60 |
|
Alternative |
Alexa Fluor 633 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
FRET |
|
Specification |
Bodipy 630/650 is part of the Bodipy family produced by Molecular Probes. These are the characteristics of Bodipy 630/650:
|
ATTO 633 [ATTO633] (DE/USA)
|
Dye |
ATTO 633 |
|
Abs |
629 |
|
Em |
657 |
|
Ext. Coeff. |
130,000 |
|
Mol. Weight |
649.00 |
|
Alternative |
LIZ |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
ATTO 633 belongs to a new generation of fluorescent labels from ATTO-TEC GmbH for the red spectral region. Characteristic features of the label are
Absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11. ATTO 633 is moderately hydrophilic. Its fluorescence is excited most efficiently in the range 610-645 nm. A suitable excitation source is the 633 nm line of the He:Ne laser or a diode-laser emitting at 635 nm. |
Alexa Fluor 647 [Alexa647] (DE/USA)
|
Dye |
Alexa Fluor 647 |
|
Abs |
650 |
|
Em |
665 |
|
Ext. Coeff. |
239,000 |
|
Mol. Weight |
957.00 |
|
Alternative |
Cy5 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Multicolour detection |
|
Specification |
The specifications from Alexa Fluor 647 from Molecular Probes are
|
Bodipy 650/665 [BO650] (DE/USA)
|
Dye |
Bodipy 650/665 |
|
Abs |
650 |
|
Em |
665 |
|
Ext. Coeff. |
101,000 |
|
Mol. Weight |
645.60 |
|
Alternative |
Cy5 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
FRET |
|
Specification |
Bodipy 650/665 is part of the Bodipy family produced by Molecular Probes. The characteristics of these modifications are
|
ATTO 647N [ATTO647N] (DE/USA)
|
Dye |
ATTO 647N |
|
Abs |
644 |
|
Em |
669 |
|
Ext. Coeff. |
150,000 |
|
Mol. Weight |
743.00 |
|
Alternative |
Cy5 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
ATTO 647N belongs to a new generation of fluorescent labels from ATTO-TEC GmbH for the red spectral region. Characteristic features of the label are
Absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. The dye is moderately hydrophilic. The fluorescence is excited most efficiently in the range 625 - 660 nm using a krypton-ion laser or a diode-laser emitting at 650 nm. |
Cyanine5 [CY5] (DE/USA)
|
Dye |
Cyanine5 |
|
Abs |
647 |
|
Em |
673 |
|
Ext. Coeff. |
250,000 |
|
Mol. Weight |
578.7 |
|
Alternative |
Quasar 670 |
|
Labelling |
5', 3', intern |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
Cyanine5 is a fluorescent dye of the cyanine family.
The fluorescence lies in the red range. |
DY-649 [DY649] (DE/USA)
|
Dye |
DY-649 |
|
Abs |
655 |
|
Em |
674 |
|
Ext. Coeff. |
250,000 |
|
Mol. Weight |
942.00 |
|
Alternative |
Quasar 670 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
DY-649 is part of the Dyomics fluorescent dyes produced by Dyomics GmbH. Dyomics dyes are characterised by
|
Cyanine5.5 [CY55] (DE/USA)
|
Dye |
Cyanine5.5 |
|
Abs |
688 |
|
Em |
711 |
|
Ext. Coeff. |
250,000 |
|
Mol. Weight |
720.9 |
|
Alternative |
Alexa Fluor 690 |
|
Labellin |
5' |
|
Purification |
HPLC |
|
Application |
Microarray experiments |
|
Specification |
Cyanine5.5 is a fluorescent dye of the cyanine phosphoramidite family. It is characterised by
Cyanine5.5 is an alternative modification in multi-colour experiments. |
ATTO 680 [ATTO680] (DE/USA)
|
Dye |
ATTO 680 |
|
Abs |
680 |
|
Em |
700 |
|
Ext. Coeff. |
125,000 |
|
Mol. Weight |
623.00 |
|
Alternative |
Alexa Fluor 680 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
ATTO 680 belongs to a new generation of fluorescent labels produced by ATTO-TEC GmbH. Characteristic features of the label are
The fluorescence is excited most efficiently in the range 645-695 nm. A suitable excitation source for the dye is the krypton-ion laser using the 676 nm line or a diode-laser emitting at 670 nm. |
DY-682 [DY682] (DE/USA)
|
Dye |
DY-682 |
|
Abs |
690 |
|
Em |
709 |
|
Ext. Coeff. |
140,000 |
|
Mol. Weight |
906.00 |
|
Alternative |
IRD 700 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
DNA sequencing |
|
Specification |
DY-682 is part of the Dyomics fluorescent dyes produced by Dyomics GmbH.
|
ATTO 700 [ATTO700] (DE/USA)
|
Dye |
ATTO 700 |
|
Abs |
700 |
|
Em |
719 |
|
Ext. Coeff. |
120,000 |
|
Mol. Weight |
663.00 |
|
Alternative |
IRD 700 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
DNA sequencing |
|
Specification |
ATTO 700 belongs to a new generation of fluorescent labels produced by ATTO-TEC GmbH. Characteristic features of the label are
ATTO 700 is a strong electron acceptor. The fluorescence is excited most efficiently in the range 670-715 nm. |
ATTO 740 [ATTO740] (DE/USA)
|
Dye |
ATTO 740 |
|
Abs |
740 |
|
Em |
764 |
|
Ext. Coeff. |
120,000 |
|
Mol. Weight |
565.00 |
|
Alternative |
Cy7 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
Real-time PCR, qPCR, FRET |
|
Specification |
ATTO 740 belongs to a new generation of fluorescent labels produced by ATTO-TEC GmbH for the near infrared spectral region. Characteristic features of the dye are
ATTO 740 is a pH sensitive product. While practically stable up to pH 7.4 (PBS-buffer), it slowly degrades at higher pH. The fluorescence is excited most efficiently in the range 720 - 755 nm. |
DY-782 [DY782] (DE/USA)
|
Dye |
DY-782 |
|
Abs |
782 |
|
Em |
800 |
|
Ext. Coeff. |
170,000 |
|
Mol. Weight |
932.00 |
|
Alternative |
IRD 800 |
|
Labelling |
5' |
|
Purification |
HPLC |
|
Application |
DNA sequencing |
|
Specification |
DY-782 is part of the Dyomics fluorescent dyes produced by Dyomics GmbH. Dyomics dyes are characterised by
|
Although the data presented in this table have been checked for accuracy, the emission, absorption and extinction coefficient values may vary slightly with each oligonucleotide.
What can you can expect from our fluorescent labelled oligos
Product specifications:
- Synthesis scales from 0.05 to 1.0 µmol
- Purification options: HPSF, HPLC (client has to take decision based on experimental requirement)
- Sequence lengths: 8-35 bases
- TAT: 2 - 3 weeks
|
Purification option |
Synthesis scale1 [µmol] |
0.05 |
0.20 |
1.00 |
|
HPSF |
Minimum yield [OD]2 |
3 |
5 |
10 |
|
HPLC |
Minimum yield [OD]2 |
2 |
3 |
6 |
1 The synthesis scale indicates the initial amount of 3'-bases
2 There is no OD guarantee for oligos <18 and >35 bases or for oligos with multiple
|
Dye |
Abs |
Em |
Ext. coeff. |
Mol. weight |
5' |
Int |
3' |
|
Alexa Fluor 350 [Alexa350] |
346 |
442 |
19,000 |
409.35 |
x |
x |
x |
|
Dy-415 [DY415] |
418 |
467 |
34,000 |
552.00 |
x |
x |
x |
|
ATTO 425 [ATTO425] |
436 |
484 |
45,000 |
499.00 |
x |
x |
x |
|
ATTO 465 [ATTO465] |
453 |
508 |
75,000 |
393.00 |
x |
x |
x |
|
Bodipy FL [BOFL] |
504 |
510 |
70,000 |
391.30 |
x |
x |
x |
|
Alexa Fluor 488 [Alexa488] |
495 |
519 |
71,000 |
630.00 |
x |
x |
x |
|
FAM [FAM] |
495 |
520 |
83,000 |
474.50 |
x |
- |
- |
|
Fluorescein isothiocyanate [FITC] |
495 |
520 |
73,000 |
505.00 |
x |
x |
x |
|
Fluorescein [FLU] |
495 |
520 |
83,000 |
504.00 |
- |
- |
x |
|
Fluorescein-dT [FLUdT] |
494 |
522 |
75,000 |
472.71 |
- |
x |
- |
|
ATTO 488 [ATTO488] |
501 |
523 |
90,000 |
687.00 |
x |
x |
x |
|
Oregon Green 488 [OG488] |
496 |
524 |
76,000 |
510.00 |
x |
x |
x |
|
Oregon Green 514 [OG514] |
506 |
526 |
85,000 |
609.40 |
x |
x |
x |
|
Rhodamine Green [RGR] |
503 |
528 |
74,000 |
472.00 |
x |
x |
X |
|
TET [TET] |
521 |
536 |
73,000 |
612.30 |
x |
- |
- |
|
ATTO 520 [ATTO520] |
516 |
538 |
110,000 |
464.00 |
x |
x |
X |
|
JOE [JOE] |
520 |
548 |
73,000 |
603.40 |
x |
x |
X |
|
Yakima Yellow [YAKYE] |
530 |
549 |
84,000 |
654.30 |
x |
- |
- |
|
Bodipy 530/550 [BO530] |
534 |
551 |
77,000 |
514.31 |
x |
x |
X |
|
HEX [HEX] |
535 |
556 |
73,000 |
681.20 |
x |
- |
- |
|
Alexa Fluor 555 [Alexa555] |
555 |
565 |
150,000 |
940.00 |
x |
x |
X |
|
Bodipy TMR-X [BOTMRX] |
544 |
570 |
56,000 |
610.55 |
x |
x |
X |
|
Cyanine3 [CY3] |
552 |
570 |
150,000 |
444.60 |
x |
- |
- |
|
ATTO 550 [ATTO550] |
554 |
576 |
120,000 |
691.00 |
x |
x |
X |
|
TAMRA [TAM] |
544 |
576 |
90,000 |
512.50 / 612.70 |
x |
x |
- |
|
Rhodamine Red [RRE] |
560 |
580 |
129,000 |
770.00 |
x |
x |
X |
|
ATTO 565 [ATTO565] |
563 |
592 |
120,000 |
608.00 |
x |
x |
X |
|
ROX [ROX] |
575 |
602 |
82,000 |
632.78 |
x |
x |
X |
|
Texas Red [TxRed] |
583 |
603 |
116,000 |
818.00 |
x |
x |
X |
|
Cyanine3.5 [CY35] |
588 |
604 |
150,000 |
544.70 |
x |
- |
- |
|
LightCycler 610 [LC610] |
590 |
610 |
n.a. |
761.48 |
x |
x |
x |
|
ATTO 594 [ATTO594] |
601 |
627 |
120,000 |
903.00 |
x |
x |
x |
|
DY-480 XL [DY480] |
500 |
630 |
50,000 |
612.68 |
x |
x |
x |
|
DY-610 [DY610] |
610 |
630 |
80,000 |
743.00 |
x |
x |
x |
|
ATTO 610 [ATTO610] |
615 |
634 |
150,000 |
488.00 |
x |
x |
x |
|
LightCycler 640 [LC640] |
625 |
640 |
n.a. |
824.68 |
x |
x |
x |
|
Bodipy 630/650 [BO630] |
625 |
640 |
101,000 |
662.60 |
x |
x |
x |
|
ATTO 633 [ATTO633] |
629 |
657 |
130,000 |
649.00 |
x |
x |
x |
|
Alexa Fluor 647 [Alexa647] |
650 |
665 |
239,000 |
957.00 |
x |
x |
x |
|
Bodipy 650/665 [BO650] |
650 |
665 |
101,000 |
645.60 |
x |
x |
x |
|
ATTO 647N [ATTO647N] |
644 |
669 |
150,000 |
743.00 |
x |
x |
x |
|
Cyanine5 [CY5] |
649 |
670 |
250,000 |
470.63 |
x |
- |
- |
|
DY-649 [DY649] |
655 |
674 |
250,000 |
942.00 |
x |
x |
x |
|
Cyanine5.5 [CY55] |
675 |
694 |
250,000 |
570.74 |
x |
- |
- |
|
ATTO 680 [ATTO680] |
680 |
700 |
125,000 |
623.00 |
x |
x |
x |
|
DY-682 [DY682] |
690 |
709 |
140,000 |
906.00 |
x |
x |
x |
|
ATTO 700 [ATTO700] |
700 |
719 |
120,000 |
663.00 |
x |
x |
x |
|
ATTO 740 [ATTO740] |
740 |
764 |
120,000 |
565.00 |
x |
x |
x |
|
DY-782 [DY782] |
782 |
800 |
170,000 |
932.00 |
x |
x |
x |
Although the data presented in this table have been checked for accuracy, the emission, absorption and extinction coefficient values may vary slightly with each oligonucleotide
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