Dark Quencher Modifications

Dark quencher extends quenching efficiency

Improve the sensitivity of your quantitative real-time PCR by using one of these quenchers:

 Dabcyl [DAB]:

Dark Quencher 400–550 nm

The Dabcyl quencher is a non-fluorescent dark quencher especially recommended for Molecular Beacons. It efficiently quenches various fluorescent dyes in the blue to green emission spectra, but it is not recommended for fluorophores emitting above 480 nm.

 Quenching Range:

400-550 nm

 Max. Absorbance:

453 nm

 Molar Extinction Coefficient:

32.000 M⁻¹ cm⁻¹

 Molecular Weight:

399.2 g/mol

 

Dark Quencher 400–550 nm

The Dabcyl quencher is a non-fluorescent dark quencher especially recommended for Molecular Beacons. It efficiently quenches various fluorescent dyes in the blue to green emission spectra, but it is not recommended for fluorophores emitting above 480 nm.

 Quenching Range:

400-550 nm

 Max. Absorbance:

453 nm

 Molar Extinction Coefficient:

32.000 M⁻¹ cm⁻¹

 Molecular Weight:

399.2 g/mol

Dabcyl-dT [DABdT]

Internal Quencher 400–550 nm

Dabcyl-dT is a non-fluorescent internal quencher. It efficiently quenches various fluorescent dyes in the blue to green emission spectra. It can be used in addition to any other 3' quencher.

 Quenching Range:

400-550 nm

 Max. Absorbance:

453 nm

 Molar Extinction Coefficient:

32.000 M⁻¹ cm⁻¹

 Molecular Weight:

399.2 g/mol

 

Internal Quencher 400–550 nm

Dabcyl-dT is a non-fluorescent internal quencher. It efficiently quenches various fluorescent dyes in the blue to green emission spectra. It can be used in addition to any other 3' quencher.

 Quenching Range:

400-550 nm

 Max. Absorbance:

453 nm

 Molar Extinction Coefficient:

32.000 M⁻¹ cm⁻¹

 Molecular Weight:

399.2 g/mol

Eclipse [Eclip]

Eclipse is a non-fluorescent dark quencher from Epoch Biosciences, Inc. This non-fluorescent dye with a maximum absorption at 522 nm effectively quenches a broad group of fluorescent dyes between emission ranges of 390–625 nm in Dual Labeled Probes and Molecular Beacons.

 Quenching Range:

390-625 nm

 Max. Absorbance:

522 nm

 Molar Extinction Coefficient:

53.000 M⁻¹ cm⁻¹

 Molecular Weight:

473.9 g/mol

 

Eclipse is a non-fluorescent dark quencher from Epoch Biosciences, Inc. This non-fluorescent dye with a maximum absorption at 522 nm effectively quenches a broad group of fluorescent dyes between emission ranges of 390–625 nm in Dual Labeled Probes and Molecular Beacons.

 Quenching Range:

390-625 nm

 Max. Absorbance:

522 nm

 Molar Extinction Coefficient:

53.000 M⁻¹ cm⁻¹

 Molecular Weight:

473.9 g/mol

BHQ1 [BHQ1]

BHQ1 is one of the Black Hole Quencher from Biosearch Technologies, Inc. BHQ1 is an highly efficient dark quencher for Dual Labeled Probes, Molecular Beacons and FRET probes with an excellent spectral overlap for all dyes in the green to dark yellow emission spectrum.

 Quenching Range:

480-580 nm

 Max. Absorbance:

534 nm

 Molar Extinction Coefficient:

34.000 M⁻¹ cm⁻¹

 Molecular Weight:

491.5 g/mol

 

BHQ1 is one of the Black Hole Quencher from Biosearch Technologies, Inc. BHQ1 is an highly efficient dark quencher for Dual Labeled Probes, Molecular Beacons and FRET probes with an excellent spectral overlap for all dyes in the green to dark yellow emission spectrum.

 Quenching Range:

480-580 nm

 Max. Absorbance:

534 nm

 Molar Extinction Coefficient:

34.000 M⁻¹ cm⁻¹

 Molecular Weight:

491.5 g/mol

BHQ1-dT [BHQ1dT]

BHQ1-dT is one of the Black Hole Quencher from Biosearch Technologies, Inc. BHQ1-dT is an highly efficient quencher used for internal labelling for all dyes in the green to dark yellow emission spectrum. It can be used in addition to other 3' quenchers.

 Quenching Range:

480-580 nm

 Max. Absorbance:

534 nm

 Molar Extinction Coefficient:

34.000 M⁻¹ cm⁻¹

 Molecular Weight:

491.5 g/mol

 

BHQ1-dT is one of the Black Hole Quencher from Biosearch Technologies, Inc. BHQ1-dT is an highly efficient quencher used for internal labelling for all dyes in the green to dark yellow emission spectrum. It can be used in addition to other 3' quenchers.

 Quenching Range:

480-580 nm

 Max. Absorbance:

534 nm

 Molar Extinction Coefficient:

34.000 M⁻¹ cm⁻¹

 Molecular Weight:

491.5 g/mol

BHQ2 [BHQ2]

BHQ2 is the second Black Hole Quencher from Biosearch Technologies, Inc. in our portfolio. BHQ2 is a highly efficient dark quencher for Dual Labeled Probes, Molecular Beacons and FRET probes containing 5’ fluorophores in the dark green to orange emission sector.

 Quenching Range:

520-650 nm

 Max. Absorbance:

544 nm

 Molar Extinction Coefficient:

91.000 M⁻¹ cm⁻¹

 Molecular Weight:

493.5 g/mol

 

BHQ2 is the second Black Hole Quencher from Biosearch Technologies, Inc. in our portfolio. BHQ2 is a highly efficient dark quencher for Dual Labeled Probes, Molecular Beacons and FRET probes containing 5’ fluorophores in the dark green to orange emission sector.

 Quenching Range:

520-650 nm

 Max. Absorbance:

544 nm

 Molar Extinction Coefficient:

91.000 M⁻¹ cm⁻¹

 Molecular Weight:

493.5 g/mol

BBQ 650 [BBQ650]

The BlackBerry Quencher 650 is an excellent long wavelength quencher for Dual Labeled Probes, Molecular Beacons and FRET probes containing long wavelength dyes such as Cy5, Cy5.5 and other dyes in the red to near-infrared emission spectrum.

 Quenching Range:

550-750 nm

 Max. Absorbance:

650 nm

 Molar Extinction Coefficient:

41.000 M⁻¹ cm⁻¹

 Molecular Weight:

575.6 g/mol