Full-length plasmid sequencing using Nanopore technology
Full-length plasmid sequencing using Nanopore technology has transformed the way plasmid DNA is characterized, enabling complete, accurate, and rapid analysis of circular DNA molecules. Unlike short-read sequencing platforms that often produce fragmented assemblies, Nanopore sequencing generates ultra-long reads that can span entire plasmid constructs in a single read. This capability allows for precise reconstruction of plasmid backbones, inserts, and repetitive or GC-rich regions that traditionally pose challenges to short-read methods.
Nanopore sequencing operates by passing individual DNA strands through nanoscale protein pores, measuring characteristic disruptions in ionic current to identify nucleotide sequences in real time. Its ability to sequence native DNA without amplification also enables the detection of base modifications, such as methylation, providing additional insight into plasmid epigenetics and regulatory function.
This approach has broad applications across synthetic biology, gene therapy, vaccine development, and biotechnology, where precise plasmid validation is essential. By providing comprehensive sequence coverage, detecting structural variants, and confirming construct integrity, Nanopore-based plasmid sequencing ensures higher reliability in research and biomanufacturing pipelines. Consequently, it represents a significant step forward in ensuring the fidelity and transparency of plasmid-based molecular research.
Full-length plasmid sequencing using Nanopore technology offered by Eurofins Genomics, provides several advantages over traditional sequencing methods.
- Fast turnaround time.
- No sequencing primers are needed.
- No PCR amplification is needed
- Cost-Effective.
- Great for GC rich or repetitive DNA
Deliverables:
- High quality reads
- Plasmid sequence
- Gene prediction
- Sanger ABI file
- Compiled Report